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[HTML] Isolation of Translating Ribosomes Containing Peptidyl-tRNAs for Functional and Structural Analyses

[HTML] from jove.comN Shirole, S Balasubramanian, C Yanofsky… - Journal of Visualized …, 2011 - jove.com
... translational complexes, well established procedures can be used, such as i)
crosslinking to specific amino acids 14 and/or ii) alkylation protection assays
1,14,17 . Files. JoVE Article PDF. Protocol. 1. Cell Free Extract Preparation. ...
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THE AN3 Protein Complex and its Use for Plant Growth Promotion

G De Jaeger, A Verkest… - US Patent 20,110,162,110, 2011 - freepatentsonline.com
... GE-Healthcare). Protein gel blots were developed by Chemiluminiscent detection
(Perkin Elmer, Norwalk, Conn.). Protein Extract Preparation. Cell material (15 g)
was grinded to homogeneity in liquid nitrogen. Crude protein ...
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An efficient factor-depleted mammalian in vitro translation system

AM Rakotondrafara… - Nature Protocols, 2011 - nature.com
... The procedure relies on RNA interference–mediated knockdown of the factor of interest prior
to extract preparation, and it overcomes problems with the availability and specificity of antibodies,
as well as with the co‐depletion of proteins associated with the factor under study. ...
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Mycobacterium tuberculosis increases IP-10 and MIG protein despite inhibition of IP-10 and MIGtranscription

X Bai, K Chmura, AR Ovrutsky, RP Bowler… - Tuberculosis, 2011 - Elsevier
... CGC TGT TGA AGT CAG AGGA-3′ (antisense). All PCR findings were normalized
to GAPDH expression. 2.6. Nuclear extract preparation and electrophoretic mobility
shift assay. THP-1 cells were infected with MTB H37Rv ...
Cited by 2 - Related articles - All 3 versions

[HTML] The protective effects of cerium oxide nanoparticles against hepatic oxidative damage induced by monocrotaline

[HTML] from nih.govKA Amin, MS Hassan, EST Awad… - International Journal of …, 2011 - ncbi.nlm.nih.gov
... after MCT administration. MCT with or without concurrent CeO 2 effects was evaluated.
Hepatic cytosolic and mitochondrial extract preparation. The liver was rinsed with
cold PBS to remove excess blood. Small portions of the ...
Cited by 1 - Related articles - All 7 versions

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