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Developmental roles for Srf, cortical cytoskeleton and cell shape in epidermal spindle orientation

C Luxenburg, HA Pasolli, SE Williams… - Nature Cell Biology, 2011 - nature.com
... (e) Immunoblots were probed with antibodies against β-actin, Wdr1 and α-tubulin. (f) Cell
morphology, pERM, cortical actin and/or myosin-IIA are perturbed in keratinocytes expressing
Actb or Wdr1 shRNAs, but not scramble control. White lines denote cell borders. ...
Cited by 1 - All 4 versions

Flow cytometric analysis of cell signaling proteins.

MA Suni… - Methods in molecular biology (Clifton, NJ), 2011 - Springer
... 12. Washing must be thorough in order to completely remove the perm buffer. Residual perm
buffer can negatively impact the staining of PE and PerCP conjugates. 13. Some cell surface
epitopes are not maintained under these fixation and permeabilization conditions. ...
Cited by 1 - All 2 versions

Immunocompetent cell functions in Ph+ acute lymphoblastic leukemia patients on prolonged Imatinib maintenance treatment

R Maggio, N Peragine, MS De Propris… - Cancer Immunology, …, 2011 - Springer
... isotypic controls. To assess cytokine production, non-adherent PBMC were analyzed
using the Fix and Perm cell permeabiliza- tion kit (Caltag Laboratories, Burlingame,
CA), according to the manufacturer's instructions. Briefly, non ...
Related articles - All 4 versions

Interplay between RAGE, CD44, and focal adhesion molecules in epithelial-mesenchymal transition of alveolar epithelial cells

ST Buckley, C Medina, M Kasper… - American Journal of …, 2011 - Am Physiological Soc
... Dr. U. Günthert, University of Basel, Institute of Pathology, Basel, Switzerland), diluted 1:10; goat
polyclonal anti-RAGE (kind gift from Dr. Michael Neeper, Merck Sharp & Dohme, West Point,
PA), diluted 1:1,000; and rabbit polyclonal anti-pERM (Cell Signaling), diluted 1:50. ...
Cited by 2 - Related articles - All 4 versions

γδT‐cell function in sepsis is modulated by C5a receptor signalling

G Han, S Geng, Y Li, G Chen, R Wang… - …, 2011 - Wiley Online Library
... staining, PE-conjugated anti-mouse CD88 antibody (C5aR; Abcam, Cambridge, MA) were washed
and used for flow cytometry analysis; for intracellular (C5L2) staining, the above cells were fixed
overnight at 4° with 1 ml fixation buffer (Fix and Perm cell permeabiliza- tion kit ...
Related articles - All 2 versions

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