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Self-inactivating helper virus for the production of high-capacity adenoviral vectors

M Gonzalez-Aparicio, I Mauleon, P Alzuguren… - Gene Therapy, 2011 - nature.com
Standard methods for producing high-capacity adenoviral vectors (HC-Ads) are based on
co-infection with a helper adenovirus (HV). To avoid HV encapsidation, its packaging signal
(Ψ) is flanked by recognition sequences for recombinases expressed in the producing ...
All 3 versions

Antibody-mediated targeted gene transfer of helper virus-free HSV-1 vectors to rat neocortical neurons that contain either NMDA receptor 2B or 2A subunits

H Cao, G Zhang… - Brain Research, 2011 - Elsevier
Because of the numerous types of neurons in the brain, and particularly the forebrain,
neuron type-specific expression will benefit many potential applications of direct gene
transfer. The two most promising approaches for achieving neuron type-specific ...

Assembly of Helper-Dependent Adenovirus DNA into Chromatin Promotes Efficient Gene Expression

PJ Ross, MA Kennedy, C Christou… - Journal of …, 2011 - Am Soc Microbiol
... hdAd-PGK-mSEAP, hdAd-lacZ, and hdAd 28E4 were amplified with the helper virus AdNG163
(46) or AdJR46 using 116 cells and standard techniques (46, 49). ... Consistent with this, we also
observed an increase in hexon at 8 hpi in cells infected with the helper virus. ...
Related articles - All 3 versions

Large-scale recombinant adeno-associated virus production

RM Kotin - Human Molecular Genetics, 2011 - Oxford Univ Press
... The AAV rep and cap genes, vg and helper virus functions are introduced efficiently into Sf9 cells
by baculovirus infection. ... S.,; Elliger C.,; Podsakoff G.,; Villarreal L.,; Kurtzman GJ,; Iwaki Y.,; Colosi
P. Adeno-associated virus vectors can be efficiently produced without helper virus.
Related articles - All 3 versions

Characterization of helper-dependent adenoviral vectors.

DJ Palmer… - Cold Spring Harbor protocols, 2011 - ncbi.nlm.nih.gov
... Palmer DJ, Ng P. INTRODUCTION This protocol describes the characterization of
helper-dependent adenoviral vectors (HDAd) with respect to physical titer, helper virus
contamination, and genomic structure using standard molecular biology techniques. ...

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