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Extreme positive selection on a new highly-expressed larval glycoprotein (LGP) gene in Galaxias fishes (Osmeriformes: Galaxiidae)

LJ Wallis… - Molecular Biology and Evolution, 2011 - SMBE
... Extreme Positive Selection on a New Highly-Expressed Larval Glycoprotein (LGP) Gene in
Galaxias Fishes (Osmeriformes: Galaxiidae). ... glycoprotein; uromodulin) and cichlid SPP120
(seminal plasma glycoprotein) due to conserved features of its zona pellucida (ZP) domain ...
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Characterization of Human Sperm Binding to Homologous Recombinant Zona Pellucida Proteins

M Chirinos, C Cariño… - Reproductive …, 2011 - rsx.sagepub.com
... 220 kDa 30 kDa Sf9 ZP4 ZP2 ZP3 SPM Primary antiserum: R anti-hSpm R pre-immune C Figure
3. Sperm binding to recombinant human zona pellucida (rhZP) proteins immobilized on a
polyvinylidene fluoride (PVDF) membrane. Serum anti-hSPM characteristics were studied. ...
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N-glycosylation of zona glycoproteins during meiotic maturation is involved in sperm-zona pellucida interactions of porcine oocytes

KM Lay, K Ashizawa, T Nakada… - Theriogenology, 2011 - Elsevier
... During fertilization, sperm initially bind to the oocyte zona pellucida (ZP), undergo the acrosome
reaction (AR), penetrate the ZP, and fuse with the oolemma to form a ... Oocytes were mounted on
a glass slide and then treated with 10 μg/mL bis-benzimide Hoechst 33342 for 10 min ...
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Alteration in the Mechanical Properties of Human Ovum Zona Pellucida Following Fertilization: Experimental and Analytical Studies

M Khalilian, M Navidbakhsh… - Experimental …, 2011 - Springer
... Introduction Zona Pellucida (ZP) is the extracellular layer of glycoprotein enveloping an oocyte. ...
55, time = 200) by using a micropipette puller (model P-97, Sutter Instrument Co., Novato, CA,
USA). The micropipette tip was cut on a microforge to obtain sharp edges. ...
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Site of the mammalian sperm physiological acrosome reaction

JM Bedford - Proceedings of the National Academy of …, 2011 - National Acad Sciences
... not only did the AR generally occur to a greater degree and often more rapidly in sperm
penetrating the cumulus than on a naked zona surface ... however, establishing the site where the
AR begins is made difficult by the fact that the egg coat—the zona pellucida—is surrounded ...
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